RNA

Part:BBa_K2781000:Design

Designed by: Liang Chenxi   Group: iGEM18_NJU-China   (2018-10-05)


TBEV 5'-UTR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There are some other endogenous UTRs of some genes, who also have similar ability to guide RNA into neurite, like the 3-'UTR of β-actin. We made comparison between these two elements and found TBEV 5'-UTR was more effective and smaller. In addition, there are two choice for delivery, exosome and virus. And we found it less effective when packed into exosomes, so we decided to use virus as a vector.

Source

This part comes from Tick-borne encephalitis virus gene, complete cds, strain:Oshima 5-10, (GenBank: AB062063.2).

References

Minato Hirano, etc. Dendritic transport of tick-borne flavivirus RNA by neuronal granules affects development of neurological disease. PNAS, 2017 , 114 (37) :9960.